Molecular diagnosis of occult bacteremia using Universal PCR method in 3 to 36 months children with fever without source and a negative blood culture

Introduction: Early diagnosis of bacteremia and its complications is the most important part of the care and management of the children. The utility of polymerase chain reaction (PCR) techniques has been shown to identify pathogens in less and more optimal time. The aim of our study was to evaluate the importance and prevalence of occult bacteremia using universal PCR of blood in 3-36 month children with fever without source in Pediatric Medical Center comparing other routine methods like blood culture. Correspondence: Narjes Ataollahi, MSc Student. Microbiology Department Jahrom University of Medical Sciences. Jahrom, Iran Tel:+98 9177615397 Email: [email protected] Methods: 100 febrile children in 3-36 month age suspected to Bacteremia with fever without source, who was admitted in children’s hospital of Bandar Abbas, were evaluated. All of them had negative blood culture. Blood samples were taken from all patients for PCR and other clinical tests such as CRP, ESR and CBC. Results: The mean temperature of the body was>39o c at presenting time. 23 patients had positive PCR test, which none of them had positive blood cultures. Laboratory findings such as WBC, ESR, and CRP had no significant difference to PCR results. Conclusion: Universal PCR technique is more sensitive and specific than conventional blood culture and other methods to diagnose organism cause bacteremia